Current Expertise | CSIR-CECRI, Karaikudi

• Microbiologically Influenced Corrosion (MIC) & Biofilms: Isolation, cultivation, and long-term strain preservation of key corrosive consortia, including Sulfate-Reducing Bacteria (SRB), Iron-Reducing Bacteria (IRB), and Manganese-Oxidizing Bacteria (MnOB); biofilm characterization and Winogradsky column enrichment models.

• Environmental Microbiology & Genomics: Metagenomic profiling via Next-Generation Sequencing (NGS); microbial ecology studies centered on bioleaching and bioremediation systems.

Past Research Expertise | IISc Bangalore & CSIR-CCMB Hyderabad

• Cell & Molecular Biology: Conventional PCR, quantitative Real-Time PCR (qPCR), molecular cloning, recombinant gene expression, and agarose/PAGE gel electrophoresis.

• Protein Biochemistry: Heterologous protein expression and induction, chromatographic separation and purification, Western blotting, immunoprecipitation (IP), SDS-PAGE/staining protocols, X-ray autoradiography, and lyophilization.

• Advanced Mammalian Cell Culture & Genome Editing: Maintenance of primary and secondary mammalian cell lines; transient and stable transfections; CRISPR-Cas9 genome editing; neural stem cell (NSC) culture and 3D brain organoid models.

• Advanced Microscopy & Imaging: Bright-field, epifluorescence, and laser scanning confocal microscopy.

• Computational Biology & Analytics: Baseline bioinformatics workflows (sequence alignment, NGS data handling, taxonomic profiling) and core biophysical analytical methods.